ELISA and RT-PCR Based Detection of Bovine Coronavirus in Northern India
نویسندگان
چکیده
منابع مشابه
Reproducibility in the quantification of mRNA levels by RT-PCR-ELISA and RT competitive-PCR-ELISA.
The use of reverse transcription (RT) PCR for relative quantitation of gene transcripts relies on the reproducibility of the individual RT, PCR and product measurement steps. Semi-competitive RT-PCR (RT-cPCR) uses an internal competitor template in the PCR step to improve quantitation. We have surveyed the reproducibility of RT, PCR, RT-cPCR and measurement, amplifying the glyceraldehyde-3-phos...
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ژورنال
عنوان ژورنال: Asian Journal of Animal and Veterinary Advances
سال: 2012
ISSN: 1683-9919
DOI: 10.3923/ajava.2012.1120.1129